Description of Assessment Task

What am I required to do in this assignment?

Brief Description of Assessment Task This assessment is made up of two tasks, a laboratory diary and a practical report. The whole weighting for this assessment is 40%. A 30% is given for your report and 10% for laboratory diary submission. A halfway through this unit (due to COVID-19), you have maintained a hand-written lab diary, including laboratory experiments which will evidence your learnings and ideas presented in the written report. You are required to prepare and submit a portfolio type report based on the provided data with the SAMPLE AND CLINICAL INFO, EXPECTED TESTS and INTERPRETATION. Laboratory reports are based on an analysis of the provided data set. Therefore, you are expected to submit a single report based on the expected tests in which you would carryout for the Identification of causative agents and you have to submit your laboratory diary by scanning your entire notebook. Guidance on the content of this report is given below & will be discussed in the tutorial.

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Submission Deadline	Marks and Feedback
Before 10am on: 05/06/2020	20 working days after deadline (L4, 5 and 7) 15 working days after deadline (L6) 10 working days after deadline (block delivery) 04/07/2020

Unit title & code	Molecular and Diagnostic Microbiology & BHS025-6
Assignment number and title	01 & Laboratory report
Assignment type	Portfolio type laboratory report
Weighting of assignment	40%
Size or length of assessment	3000
Unit learning outcomes	1 Demonstrate the following knowledge and understanding Detailed knowledge of the scientific basis of molecular and diagnostic microbiology, including current research and applications. 2 Demonstrate the following skills and abilities Accurately report, analyse and interpret data in molecular and diagnostic microbiology

Marks and feedback
Completing Your Assignment
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Skills taught / practiced / assessed
Core technical skills
Biosafety &
containment.
Aseptic technique.
Pipetting.
Sample handling.
Use of basic lab
equipment.
Liquid handling.
Subject-specific skills
Diagnosis methods.
Streak plating.
Use of selective and
differential agar.
Biochemical
identification.
Molecular typing-16S
rRNA
Transferable skills
Time management. Team
work. Communication.
Scientific writing.
Literature searching.
Word processing. Data
presentation.
Referencing.
Decision making.
Critical analysis and
interpretation
Maths / Statistics
Data handling. Scientific
notation.

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What do I need to do to pass? (Threshold Expectations from UIF)

In order to pass Assessment 1 you will need to: Carry-out investigatory work to solve a problem relating to the diagnosis of infectious disease, evidenced in a research diary/notebook, completed according to the assignment brief, which accurately records experimental aims, methods, findings and conclusions. Analyse and present data in an appropriate format within a written report according to the format specified in the assignment brief.

How do I produce high quality work that merits a good grade?

Structure of the report Your assignment should be formatted as a portfolio (a single document) of 6 clinical microbiology reports. This means you write one short report for each case study. EACH SHORT REPORT SHOULD NOT EXCEED 500 WORDS, so your whole portfolio will not exceed 3000 words (excluding tables & references). See BREO for an example of the layout. Each section should be clearly labelled. Clarity of English and presentation is essential throughout. 1. SAMPLE AND CLINICAL INFORMATION: • What is the likely disease which is presented in the case study? Identify the disease and analyze the symptoms with the disease • What kind of sample do you request for the identification? Say Blood or urine or sputum sample • What is the sample collection technique routinely employed? Discuss the sample collection technique and state the sample volume, sampling time, sampling container, patient preparation etc. • Present sample type and disease • Provide the sample collection procedure relevant to each sample

• How would you process this sample?
Discuss direct microscopy if necessary, inoculating into an enrichment broth, plating on selective and differential media.

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• Highlight a key detail for each sample that you think is likely to be an important factor in the case.
2. EXPECTED TEST RESULTS:
Ensure that you include information for each relevant test for each organism. As this is a short report used to convey
information, you may not need to write full paragraphs of text. Present in a table (Table not included in the word count) and
summarise the table in a couple of sentences.
Your results include:
conventional tests, simple biochemical tests, API and molecular tests.
The data can be presented in a table. Please note the table and references are not included in the word count.

Tests	Expected results
Sample nature & observation
Direct Microscopy if necessary
Gram staining
Culture characteristics on selective and differential media
Phenotypic identification
Hemolytic activity if necessary
Simple biochemical tests
Conventional TSI if necessary
API tests
Molecular tests if any

• 3) INTERPRETATION (minimum 250 words in each individual report) • You should discuss the meaning of your findings here in full paragraphs of text. Ensure that you relate the identity of each organism to the symptoms of the disease. You MUST: • suggest what disease each patient could be suffering based on the organism isolated from the patient • highlight any risk factors or possible underlying issues in the patient profile, explaining WHY these could contribute to disease caused by the particular organism • for each microorganism, give at least two key relevant virulence factors and explain how these enhance pathogenicity • suggest any automation system that may be performed for the identification.

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• Finally for each organism, write a report and suggest a suitable treatment based on your AST data Your discussion should provide evidence of your ability to evaluate the role of pathogens and their pathogenicity in disease and illness. 4) References / Bibliography You MUST include a list of references, in the UoB/Harvard format (see in a single reference list.) https://lrweb.beds.ac.uk/__data/assets/pdf_file/0009/557568/UoBHarvard17_18.pdf). Ensure that all of your references are also cited in your text at the appropriate point. Aim to include at least 10 references, using no more than two textbooks. You MUST NOT copy any text from any sources, even if you cite the source – you must write about what you have read in your own words – this is very important. There should be a single reference list section listing all references cited in both parts of the report. You should include peer-reviewed scientific journal articles or textbooks as sources. These should be listed in the correct University of Bedfordshire Harvard Referencing format in a single reference list (see here for details: https://lrweb.beds.ac.uk/__data/assets/pdf_file/0009/557568/UoBHarvard17_18.pdf). The reference list should only contain sources that have been cited appropriately – e.g. (Smith, 2010) – in the main text of your report.

How does this assignment relate to what we are doing in scheduled sessions?

At Molecular and Diagnostic Microbiology unit lectures, we cover all the theoretical aspects of diagnostic Microbiology techniques. The lectures will focus on sample collection, processing on selective media and wet mount investigation, identification using simple Biochemical tests including conventional tests and API tests, molecular tests and finally AST.

How will my assignment be marked?
Your assignment will be marked according to the threshold expectations and the criteria on the following page. You can use them to evaluate your own work and consider your grade before you submit.
Pass – 40-49%	Pass – 50-59%	Commendation – 60- 69%	Distinction– 70%+
1 Quality of understanding and analysis of scientific principles and	Acceptable level of understanding of relevant scientific principles and knowledge base.	Good level of understanding of relevant scientific principles and knowledge base. Adequate review of relevant	Very high level of understanding of relevant scientific principles and knowledge base. A very good review of relevant	A comprehensive understanding of the scientific principles and knowledge base. Detailed and focused review of

Marks and Feedback
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knowledge base.	Satisfactory review of relevant literature, though some omissions or tangents. A reasonable attempt to relate study to broader context and explain aim and approach.	literature. A good attempt to relate study to broader context and explain aim and approach.	literature. A very good attempt to relate study to broader context and explain aim and approach.	previously published literature. Broader context of study clearly described. Experimental aim and approach well defined.
2 Data handling and presentation.	Satisfactory data analysis. Presentation is generally clear and appropriate. Some attempt is given to explain what is being presented.	Good data analysis. Presentation is generally clear and appropriate. Some attempt is given to explain what is being presented.	Very good data analysis and is accurate, thorough and complete. Presentation is clear and appropriate. Clear explanation of what is presented is given. Sound understanding of data analysis shown.	Excellent data analysis and is accurate, thorough and complete. Presentation is clear and appropriate. Clear explanation of what is presented is given. Sound understanding of data analysis shown.
3 Critical evaluation and discussion.	Satisfactory evidence of reflection and evaluation of scientific problem and approach. Some critical evaluation of cited literature, though at times a little shallow. Demonstrates some ability to make evaluative links between the current scientific thought and the work in hand, but the evaluation is sometimes rather superficial.	Good evidence of reflection and evaluation of scientific problem and approach. Good critical evaluation of cited literature. Demonstrates good ability to make evaluative links between the current scientific thought and the work in hand.	Demonstrates an enhanced ability to evaluate scientific problems and to make clear evaluative links between the current scientific thought and the work in hand, which are capable of contributing to the advance of scientific knowledge. Shows critical evaluation of cited literature.	Demonstrates an excellent ability to evaluate scientific problems and to make clear evaluative links between the current scientific thought and the work in hand, which are capable of contributing to the advance of scientific knowledge. Shows deep critical evaluation of cited literature.
4 Written expression and structure.	Satisfactory written expression and satisfactory level of presentation. Planning could have been more thorough in parts.	Written expression is clear and arguments can be followed without undue difficulty. The structure of the work is appropriate.	Written expression is clear and concise. Arguments are put forward succinctly and the structure of the piece is well-planned, well-thought out and logical, enhancing its readability.	An excellent Written expression and is clear and concise. Arguments are put forward succinctly and the structure of the piece is well-planned, well-thought out and logical, enhancing its readability.
5 Use of literature and referencing.	A satisfactory range of literature is accessed, including primary sources. In-text citations are used appropriately and UoB Harvard style format is	A good range of literature is accessed, including important primary sources. In-text citations are used appropriately and UoB Harvard style format is generally correctly used.	A wide range of literature is accessed, including important primary sources. In-text citations are used appropriately and UoB Harvard style format is generally correctly used.	A broad range of primary sources is accessed. Correct UoB Harvard style formatting of citations and reference list used throughout.

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generally correctly used.

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